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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Nucleolin/C23 Polyclonal antibody (10556-1-AP)

    WB analysis using 10556-1-AP (same clone as 10556-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10556-1-AP (Nucleolin/C23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10556-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using Nucleolin/C23 Polyclonal antibody (10556-1-AP)

    WB analysis of rat brain using 10556-1-AP (same clone as 10556-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 10556-1-AP (Nucleolin/C23 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 10556-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using Nucleolin/C23 Polyclonal antibody (10556-1-AP)

    IP experiment of HeLa using 10556-1-AP (same clone as 10556-1-PBS)

    IP result of anti-Nucleolin/C23 (IP:10556-1-AP, 4ug; Detection:10556-1-AP 1:1000) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 10556-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Nucleolin/C23 Polyclonal antibody (10556-1-AP)

    IHC staining of human lung cancer using 10556-1-AP (same clone as 10556-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10556-1-AP (Nucleolin/C23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10556-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using NCL Polyclonal antibody, PBS Only (10556-1-PBS)

    IF Staining of HEK-293 using 10556-1-PBS

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NCL antibody (10556-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using NCL Polyclonal antibody, PBS Only (10556-1-PBS)

    IF Staining of HEK-293 using 10556-1-PBS

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NCL antibody (10556-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using Nucleolin/C23 Polyclonal antibody (10556-1-AP)

    IF Staining of HEK-293 using 10556-1-AP (same clone as 10556-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NCL antibody (10556-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 10556-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Nucleolin/C23 Polyclonal antibody, PBS Only

Nucleolin/C23 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10556-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

C23, nucleolin, FLJ45706, NCL, Protein C23

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10556-1-PBS targets Nucleolin/C23 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0859

Product name: Recombinant human NCL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-482 aa of BC006494

Sequence: MVKLAKAGKNQGDPKKMAPPPKEVEEDSEDEEMSEDEEDDSSGEEVVIPQKKGKKAAATSAKKVVVSPTKKVAVATPAKKAAVTPGKKAAATPAKKTVTPAKAVTTPGKKGATPGKALVATPGKKGAAIPAKGAKNGKNAKKEDSDEEEDDDSEEDEEDDEDEDEDEDEIEPAAMKAAAAAPASEDEDDEDDEDDEDGKSKGIAYIEFKTEADAEKTFEEKQGTEIDGRSISLYYTGEKGQNQDYRGGKNSTWSGESKTLVLSNLSYSATEETLQEVFEKATFIKVPQNQNGKSKGYAFIEFASFEDAKEALNSCNKREIEGRAIRLELQGPRGSPNARSQPSKTLFVKGLSEDTTEETLKESFDGSVRARIVTDRETGSSKGFGFVDFNSEEDAKAAKEAMEDGEIDGNKVTLDWAKPKGEGGFGGRGGGRGGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDHKPQGKKTKFE

相同性解析による交差性が予測される生物種
Full Name nucleolin
Calculated molecular weight 76 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC006494
Gene Symbol NCL
Gene ID (NCBI) 4691
RRIDAB_2082423
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP19338
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Nucleolin, also known as C23, involved in the control of transcription of ribosomal RNA (rRNA) genes by RNA polymerase I, in nucleocytoplasmic transportation of ribosomal components, and in ribosome maturation and assembly. It associated with intranucleolar chromatin and pre-ribosomal particles, and induced chromatin decondensation by binding to histione H1. Also it has a role in the process of transcriptional elongation. Whilst mammalian nucleolin has a predicted molecular mass of approximately 77 kDa, the apparent molecular mass is between 100 and 110 kDa, and has been attributed to the amino acid composition of the N-terminal domain, which is highly phosphorylated. (PMID: 15925566)

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