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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NCBP3 Polyclonal antibody (24384-1-AP)

    WB analysis using 24384-1-AP (same clone as 24384-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24384-1-AP (NCBP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24384-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NCBP3 Polyclonal antibody (24384-1-AP)

    IF Staining of HeLa using 24384-1-AP (same clone as 24384-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NCBP3 antibody (24384-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 24384-1-PBS in a different storage buffer formulation.

NCBP3 Polyclonal antibody, PBS Only

NCBP3 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 24384-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

C17orf85, ELG, HSA277841, Nuclear cap-binding protein subunit 3, Protein ELG

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24384-1-PBS targets NCBP3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20021

Product name: Recombinant human C17orf85 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 16-340 aa of BC148856

Sequence: SNSWKRRYHSRRIQRDVIKKRALIGDDVGLTSYKHRHSGLVNVPEEPIEEEEEEEEEEEEEEEEDQDMDADDRVVVEYHEELPALKQPRERSASRRSSASSSDSDEMDYDLELKMISTPSPKKSMKMTMYADEVESQLKNIRNSMRADSVSSSNIKNRIGNKLPPEKFADVRHLLDEKRQHSRPRPPVSSTKSDIRQRLGKRPHSPEKAFSSNPVVRREPSSDVHSRLGVPRQDSKGLYADTREKKSGNLWTRLGSAPKTKEKNTKKVDHRAPGAEEDDSELQRAWGALIKEKEQSRQKKSRLDNLPSLQIEVSRESSSGSEAES

相同性解析による交差性が予測される生物種
Full Name chromosome 17 open reading frame 85
Calculated molecular weight 620 aa, 70 kDa
Observed molecular weight 71-80 kDa
GenBank accession numberBC148856
Gene Symbol NCBP3/C17orf85
Gene ID (NCBI) 55421
RRIDAB_2879518
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53F19
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

chromosome 17 open reading frame 85(C17orf85)also named as Protein ELG is 620 amino acids protein, which is localizes in human chromosome 17. The function of protein is still non-known and it consists of some phosphorylation site . It may involves in human embryonic stem cell differentiation.

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