Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, Jurkat cells, PC-3 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
24384-1-AP targets NCBP3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20021 Product name: Recombinant human C17orf85 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 16-340 aa of BC148856 Sequence: SNSWKRRYHSRRIQRDVIKKRALIGDDVGLTSYKHRHSGLVNVPEEPIEEEEEEEEEEEEEEEEDQDMDADDRVVVEYHEELPALKQPRERSASRRSSASSSDSDEMDYDLELKMISTPSPKKSMKMTMYADEVESQLKNIRNSMRADSVSSSNIKNRIGNKLPPEKFADVRHLLDEKRQHSRPRPPVSSTKSDIRQRLGKRPHSPEKAFSSNPVVRREPSSDVHSRLGVPRQDSKGLYADTREKKSGNLWTRLGSAPKTKEKNTKKVDHRAPGAEEDDSELQRAWGALIKEKEQSRQKKSRLDNLPSLQIEVSRESSSGSEAES 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 17 open reading frame 85 |
| Calculated molecular weight | 620 aa, 70 kDa |
| Observed molecular weight | 71-80 kDa |
| GenBank accession number | BC148856 |
| Gene Symbol | NCBP3/C17orf85 |
| Gene ID (NCBI) | 55421 |
| RRID | AB_2879518 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q53F19 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
chromosome 17 open reading frame 85(C17orf85)also named as Protein ELG is 620 amino acids protein, which is localizes in human chromosome 17. The function of protein is still non-known and it consists of some phosphorylation site . It may involves in human embryonic stem cell differentiation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NCBP3 antibody 24384-1-AP | Download protocol |
| IF protocol for NCBP3 antibody 24384-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |