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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NAT10 Monoclonal antibody (67465-1-Ig)

    WB analysis using 67465-1-Ig (same clone as 67465-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67465-1-Ig (NAT10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67465-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using NAT10 Monoclonal antibody (67465-1-Ig)

    WB analysis of A549 using 67465-1-Ig (same clone as 67465-1-PBS)

    WB result of NAT10 antibody (67465-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAT10 transfected A549 cells. This data was developed using the same antibody clone with 67465-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NAT10 Monoclonal antibody (67465-1-Ig)

    WB analysis of HeLa using 67465-1-Ig (same clone as 67465-1-PBS)

    WB result of NAT10 antibody (67465-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAT10 transfected HeLa cells. This data was developed using the same antibody clone with 67465-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NAT10 Monoclonal antibody, PBS Only

NAT10 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67465-1-PBS
Clone No.3E3D8

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

18S rRNA cytosine acetyltransferase, 3E3D8, ALP, EC:2.3.1.-, KIAA1709

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67465-1-PBS targets NAT10 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag4355

Product name: Recombinant human NAT10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 676-1025 aa of BC035558

Sequence: EAVSLLEEVITPRKDLPPLLLKLNERPAERLDYLGVSYGLTPRLLKFWKRAGFVPVYLRQTPNDLTGEHSCIMLKTLTDEDEADQGGWLAAFWKDFRRRFLALLSYQFSTFSPSLALNIIQNRNMGKPAQPALSREELEALFLPYDLKRLEMYSRNMVDYHLIMDMIPAISRIYFLNQLGDLALSAAQSALLLGIGLQHKSVDQLEKEIELPSGQLMGLFNRIIRKVVKLFNEVQEKAIEEQMVAAKDVVMEPTMKTLSDDLDEAAKEFQEKHKKEVGKLKSMDLSEYIIRGDDEEWNEVLNKAGPNASIISLKSDKKRKLEAKQEPKQSKKLKNRETKNKKDMKLKRKK

相同性解析による交差性が予測される生物種
Full Name N-acetyltransferase 10 (GCN5-related)
Calculated molecular weight 1025 aa, 116 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC035558
Gene Symbol NAT10
Gene ID (NCBI) 55226
RRIDAB_2882694
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9H0A0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NAT10 (N-acetyltransferase 10) is a nucleolar protein that is involved in regulation of telomerase activity, DNA damage response, and cytokinesis. It also plays a role in maintaining nuclear shape. Inhibition of NAT10 has been reported to rescue the misshapen nuclei in laminopathic cells via microtubule reorganization.