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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NAT10 Monoclonal antibody (67465-1-Ig)

    WB analysis using 67465-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67465-1-Ig (NAT10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using NAT10 Monoclonal antibody (67465-1-Ig)

    WB analysis of A549 using 67465-1-Ig

    WB result of NAT10 antibody (67465-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAT10 transfected A549 cells.

  • Western Blot (WB) analysis of HeLa cells using NAT10 Monoclonal antibody (67465-1-Ig)

    WB analysis of HeLa using 67465-1-Ig

    WB result of NAT10 antibody (67465-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAT10 transfected HeLa cells.

  • Featured Product
  • KD/KO Validated

NAT10 Monoclonal antibody

NAT10 Monoclonal Antibody for WB, ELISA
Cat No. 67465-1-Ig
Clone No.3E3D8

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

18S rRNA cytosine acetyltransferase, 3E3D8, ALP, EC:2.3.1.-, KIAA1709

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, A549 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, Neuro-2a cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67465-1-Ig targets NAT10 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag4355

Product name: Recombinant human NAT10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 676-1025 aa of BC035558

Sequence: EAVSLLEEVITPRKDLPPLLLKLNERPAERLDYLGVSYGLTPRLLKFWKRAGFVPVYLRQTPNDLTGEHSCIMLKTLTDEDEADQGGWLAAFWKDFRRRFLALLSYQFSTFSPSLALNIIQNRNMGKPAQPALSREELEALFLPYDLKRLEMYSRNMVDYHLIMDMIPAISRIYFLNQLGDLALSAAQSALLLGIGLQHKSVDQLEKEIELPSGQLMGLFNRIIRKVVKLFNEVQEKAIEEQMVAAKDVVMEPTMKTLSDDLDEAAKEFQEKHKKEVGKLKSMDLSEYIIRGDDEEWNEVLNKAGPNASIISLKSDKKRKLEAKQEPKQSKKLKNRETKNKKDMKLKRKK

相同性解析による交差性が予測される生物種
Full Name N-acetyltransferase 10 (GCN5-related)
Calculated molecular weight 1025 aa, 116 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC035558
Gene Symbol NAT10
Gene ID (NCBI) 55226
RRIDAB_2882694
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9H0A0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NAT10 (N-acetyltransferase 10) is a nucleolar protein that is involved in regulation of telomerase activity, DNA damage response, and cytokinesis. It also plays a role in maintaining nuclear shape. Inhibition of NAT10 has been reported to rescue the misshapen nuclei in laminopathic cells via microtubule reorganization.

Protocols

Product Specific Protocols
WB protocol for NAT10 antibody 67465-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols