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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Muskelin Polyclonal antibody (14735-1-AP)

    WB analysis using 14735-1-AP (same clone as 14735-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14735-1-AP (Muskelin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14735-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Y79 cells using Muskelin Polyclonal antibody (14735-1-AP)

    WB analysis of Y79 using 14735-1-AP (same clone as 14735-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 14735-1-AP (Muskelin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14735-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using Muskelin Polyclonal antibody (14735-1-AP)

    WB analysis of Jurkat using 14735-1-AP (same clone as 14735-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14735-1-AP (Muskelin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14735-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Muskelin Polyclonal antibody (14735-1-AP)

    IHC staining of human gliomas using 14735-1-AP (same clone as 14735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 14735-1-AP (Muskelin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14735-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Muskelin Polyclonal antibody (14735-1-AP)

    IHC staining of human gliomas using 14735-1-AP (same clone as 14735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 14735-1-AP (Muskelin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14735-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Muskelin Polyclonal antibody, PBS Only

Muskelin Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14735-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

MKLN1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14735-1-PBS targets Muskelin in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6147

Product name: Recombinant human MKLN1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 388-735 aa of BC067825

Sequence: FDHQMCMDSEKHMIYTFGGRILTCNGSVDDSRASEPQFSGLFAFNCQCQTWKLLREDSCNAGPEDIQSRIGHCMLFHSKNRCLYVFGGQRSKTYLNDFFSYDVDSDHVDIISDGTKKDSGMVPMTGFTQRATIDPELNEIHVLSGLSKDKEKREENVRNSFWIYDIVRNSWSCVYKNDQAAKDNPTKSLQEEEPCPRFAHQLVYDELHKVHYLFGGNPGKSCSPKMRLDDFWSLKLCRPSKDYLLRHCKYLIRKHRFEEKAQVDPLSALKYLQNDLYITVDHSDPEETKEFQLLASALFKSGSDFTALGFSDVDHTYAQRTQLFDTLVNFFPDSMTPPKGNLVDLITL

相同性解析による交差性が予測される生物種
Full Name muskelin 1, intracellular mediator containing kelch motifs
Calculated molecular weight 735 aa, 85 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC067825
Gene Symbol Muskelin
Gene ID (NCBI) 4289
RRIDAB_2142504
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UL63
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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