Validation Data Gallery

Filter: AllWBIHC

WB analysis of Y79 using 14735-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 14735-1-AP (Muskelin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 14735-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 14735-1-AP (Muskelin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 14735-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 14735-1-AP (Muskelin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 14735-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 14735-1-AP (Muskelin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 14735-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 14735-1-AP (Muskelin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human gliomas using 14735-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas using 14735-1-AP (Muskelin antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human gliomas using 14735-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas using 14735-1-AP (Muskelin antibody) at dilution of 1:50 (under 10x lens).

Featured Product
KD/KO Validated

Muskelin Polyclonal antibody

Muskelin Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14735-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

MKLN1, Muskelin, TWA2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Y79 cells, Jurkat cells
Positive IHC detected in	human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 4 publications below
IF	See 1 publications below
IP	See 2 publications below

Product Information

14735-1-AP targets Muskelin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6147 Product name: Recombinant human MKLN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 388-735 aa of BC067825 Sequence: FDHQMCMDSEKHMIYTFGGRILTCNGSVDDSRASEPQFSGLFAFNCQCQTWKLLREDSCNAGPEDIQSRIGHCMLFHSKNRCLYVFGGQRSKTYLNDFFSYDVDSDHVDIISDGTKKDSGMVPMTGFTQRATIDPELNEIHVLSGLSKDKEKREENVRNSFWIYDIVRNSWSCVYKNDQAAKDNPTKSLQEEEPCPRFAHQLVYDELHKVHYLFGGNPGKSCSPKMRLDDFWSLKLCRPSKDYLLRHCKYLIRKHRFEEKAQVDPLSALKYLQNDLYITVDHSDPEETKEFQLLASALFKSGSDFTALGFSDVDHTYAQRTQLFDTLVNFFPDSMTPPKGNLVDLITL 相同性解析による交差性が予測される生物種
Full Name	muskelin 1, intracellular mediator containing kelch motifs
Calculated molecular weight	735 aa, 85 kDa
Observed molecular weight	70-75 kDa
GenBank accession number	BC067825
Gene Symbol	Muskelin
Gene ID (NCBI)	4289
RRID	AB_2142504
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UL63
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for Muskelin antibody 14735-1-AP	Download protocol
WB protocol for Muskelin antibody 14735-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IP,IF	Neuron Muskelin Coordinates PrPC Lysosome versus Exosome Targeting and Impacts Prion Disease Progression. Authors - Frank F Heisler KO Validated View Article
human	WB	Sci Rep Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin. Authors - Shimaa H A Soliman View Article
human	WB,IP	Mol Cell mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway Authors - Sang Ah Yi KO Validated View Article
human	WB	Cell Rep mTORC1 regulates the pyrimidine salvage pathway by controlling UCK2 turnover via the CTLH-WDR26 E3 ligase Authors - Brittany Q Pham View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Y79 using 14735-1-AP

WB analysis of Y79 using 14735-1-AP

WB analysis of Jurkat using 14735-1-AP

WB analysis of Jurkat using 14735-1-AP

WB analysis of Y79 using 14735-1-AP

IHC staining of human gliomas using 14735-1-AP

IHC staining of human gliomas using 14735-1-AP

Muskelin Polyclonal antibody

Muskelin Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Muskelin Coordinates PrPC Lysosome versus Exosome Targeting and Impacts Prion Disease Progression.

Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin.

mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway

mTORC1 regulates the pyrimidine salvage pathway by controlling UCK2 turnover via the CTLH-WDR26 E3 ligase