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  • Featured Product
  • KD/KO Validated

Muskelin Polyclonal antibody

Muskelin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14735-1-AP

Synonyms

MKLN1, Muskelin, TWA2

Validation Data Gallery

Applications:
  • WB analysis of Y79 using 14735-1-AP

    WB analysis of Y79 using 14735-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 14735-1-AP (Muskelin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of Y79 using 14735-1-AP

    WB analysis of Y79 using 14735-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 14735-1-AP (Muskelin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of Y79 using 14735-1-AP

    WB analysis of Y79 using 14735-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 14735-1-AP (Muskelin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 14735-1-AP

    WB analysis of Jurkat using 14735-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14735-1-AP (Muskelin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 14735-1-AP

    WB analysis of Jurkat using 14735-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14735-1-AP (Muskelin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 14735-1-AP

    IHC staining of human gliomas using 14735-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 14735-1-AP (Muskelin antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human gliomas using 14735-1-AP

    IHC staining of human gliomas using 14735-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 14735-1-AP (Muskelin antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inY79 cells, Jurkat cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14735-1-AP targets Muskelin in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Muskelin fusion protein Ag6147 相同性解析による交差性が予測される生物種
Full Name muskelin 1, intracellular mediator containing kelch motifs
Calculated molecular weight 735 aa, 85 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC067825
Gene symbol MKLN1
Gene ID (NCBI) 4289
RRIDAB_2142504
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Muskelin antibody 14735-1-APDownload protocol
IHC protocol for Muskelin antibody 14735-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP,IF

Neuron

Muskelin Coordinates PrPC Lysosome versus Exosome Targeting and Impacts Prion Disease Progression.

Authors - Frank F Heisler
  • KO Validated
View Article
humanWB

Sci Rep

Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin.

Authors - Shimaa H A Soliman
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

S (Verified Customer) (01-01-2022)

This antibody is ok, but not the best. At least, KO validation. The weak signal could be due to the low protein expression level though.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T and several others
Muskelin Antibody Western Blot validation (1:1000 dilution) in HEK293T and several others (Cat no:14735-1-AP)
FH

H (Verified Customer) (04-05-2020)

Primary antibody: 1:1000 dilution in 5% BSA, over night at 4CSecondary antibody: Rabbit IRDye800Detection: odyssey ClxI was able to detect the band in HEK293T and HCT116 cells. However, the band intensity was not that strong.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
Muskelin Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:14735-1-AP)