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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse IgG2a isotype control Monoclonal antibody, PBS Only

Mouse IgG2a isotype control Monoclonal Antibody for WB, FC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Megathura crenulata

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

11A1B2

Cat no : 66360-2-PBS

Synonyms

Mouse IgG2a, Mouse IgG2a isotype control

Validation Data Gallery

Applications:
  • WB analysis using 66360-2-Ig (same clone as 66360-2-PBS)

    WB analysis using 66360-2-Ig (same clone as 66360-2-PBS)

    Various lysates (50 μg/lane) were subjected to SDS PAGE followed by western blot with 66360-2-Ig (Mouse IgG2a isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66360-2-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66360-2-Ig (same clone as 66360-2-PBS)

    FC experiment of HepG2 using 66360-2-Ig (same clone as 66360-2-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Mouse IgG2a isotype control (66360-2-Ig, Clone: 11A1B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Cytochrome c Mouse McAb (66264-1-Ig, Clone: 2D8D11) (green) or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66360-2-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

66360-2-PBS targets Mouse IgG2a isotype control in WB, FC, ELISA applications and shows reactivity with Megathura crenulata samples.

Tested Reactivity Megathura crenulata
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name Mouse IgG2a isotype control
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This antibody is used as the isotype control of mouse IgG2a. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).