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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse IgG2a isotype control Monoclonal antibody

Mouse IgG2a isotype control Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Megathura crenulata

Applications

WB, IP, FC, ELISA

Conjugate

Unconjugated

CloneNo.

11A1B2

Cat no : 66360-2-Ig

Synonyms

Mouse IgG2a, Mouse IgG2a isotype control

"Mouse IgG2a isotype control Antibodies" Comparison

View side-by-side comparison of Mouse IgG2a isotype control antibodies from other vendors to find the one that best suits your research needs.
Applications:
  • WB analysis using 66360-2-Ig

    WB analysis using 66360-2-Ig

    Various lysates (50 μg/lane) were subjected to SDS PAGE followed by western blot with 66360-2-Ig (Mouse IgG2a isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 66360-2-Ig

    FC experiment of HepG2 using 66360-2-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Mouse IgG2a isotype control (66360-2-Ig, Clone: 11A1B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Cytochrome c Mouse McAb (66264-1-Ig, Clone: 2D8D11) (green) or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected in
Positive FC detected in

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

66360-2-Ig targets Mouse IgG2a isotype control in WB, IP, FC, ELISA applications and shows reactivity with Megathura crenulata samples.

Tested Reactivity Megathura crenulata
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name Mouse IgG2a isotype control
Gene symbol
Gene ID (NCBI)
RRIDAB_2881739
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This antibody is used as the isotype control of mouse IgG2a. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

Protocols

Product Specific Protocols
WB protocol for Mouse IgG2a isotype control antibody 66360-2-IgDownload protocol
FC protocol for Mouse IgG2a isotype control antibody 66360-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
FC

Biosens Bioelectron

Spherical nucleic acids-based cascade signal amplification for highly sensitive detection of exosomes.

Authors - Lei Wang
View Article
IP

Nature

CDK11 regulates pre-mRNA splicing by phosphorylation of SF3B1

Authors - Milan Hluchý
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Niklas (Verified Customer) (07-31-2023)

better staining control than secondary only control

  • Applications: Immunofluorescence, Flow Cytometry
  • Primary Antibody Dilution: 1:200-1:400
  • Cell Tissue Type: mouse and human heart