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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Mmp9 Polyclonal antibody (30592-1-AP)

    WB analysis using 30592-1-AP (same clone as 30592-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30592-1-AP (Mmp9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30592-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using Mmp9 Polyclonal antibody (30592-1-AP)

    IP experiment of mouse lung using 30592-1-AP (same clone as 30592-1-PBS)

    IP result of anti-Mmp9 (IP:30592-1-AP, 4ug; Detection:30592-1-AP 1:6000) with mouse lung tissue lysate 1200 ug. This data was developed using the same antibody clone with 30592-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Mmp9 Polyclonal antibody (30592-1-AP)

    IF Staining of A431 using 30592-1-AP (same clone as 30592-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Mmp9 antibody (30592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30592-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using Mmp9 Polyclonal antibody (30592-1-AP)

    IF Staining of U2OS using 30592-1-AP (same clone as 30592-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Mmp9 antibody (30592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30592-1-PBS in a different storage buffer formulation.

Mouse Mmp9 Polyclonal antibody, PBS Only

Mmp9 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 30592-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30592-1-PBS targets Mmp9 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Recombinant protein

相同性解析による交差性が予測される生物種
Full Name matrix metallopeptidase 9
Calculated molecular weight81kd
Observed molecular weight80-100 kDa
GenBank accession numberNM_013599
Gene Symbol Mmp9
Gene ID (NCBI) 17395
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP41245-1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Mmp9 (matrix metallopeptidase 9), also known as Clg4b. It is expected to be located inextracellular matrix. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The calculated molecular weight of Mmp9 is 80 kDa, the pro-MMP9 is 92 kDa, and this protein can exist as a dimer of 180 kDa (PMID:7492685).