Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse lung tissue, mouse spleen tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IF/ICC detected in | U2OS cells, A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 14 publications below |
| IF | See 2 publications below |
Product Information
30592-1-AP targets Mmp9 in WB, IF/ICC, IP, ELISA applications and shows reactivity with mouse, human samples.
| Tested Reactivity | mouse, human |
| Cited Reactivity | human, mouse, rat, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | matrix metallopeptidase 9 |
| Calculated molecular weight | 81kd |
| Observed molecular weight | 80-100 kDa |
| GenBank accession number | NM_013599 |
| Gene Symbol | Mmp9 |
| Gene ID (NCBI) | 17395 |
| RRID | AB_3086370 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P41245-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Mmp9 (matrix metallopeptidase 9), also known as Clg4b. It is expected to be located inextracellular matrix. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The calculated molecular weight of Mmp9 is 80 kDa, the pro-MMP9 is 92 kDa, and this protein can exist as a dimer of 180 kDa (PMID:7492685).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Mmp9 antibody 30592-1-AP | Download protocol |
| IP protocol for Mmp9 antibody 30592-1-AP | Download protocol |
| WB protocol for Mmp9 antibody 30592-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Small A Constant Filgotinib Delivery Adhesive Platform Based on Polyethylene Glycol (PEG) Hydrogel for Accelerating Wound Healing via Restoring Macrophage Mitochondrial Homeostasis | ||
Int J Mol Sci Impact of Escherichia coli and Lipopolysaccharide on the MAPK Signaling Pathway, MMPs, TIMPs, and the uPA System in Bovine Mammary Epithelial Cells | ||
Ecotoxicol Environ Saf Peptide CyRL-QN15 alleviates UVB-induced skin aging by promoting the repair of barrier | ||
Acta Biomater A costal-cartilage derived stem cell-laden prominin-1-derived peptide collagen hydrogel for angiogenesis and bone regeneration | ||
Front Toxicol Tributyl phosphate as a type of environmental endocrine disruptor associated with liver fibrosis: insights from NHANES and in vitro validation | ||
Front Pharmacol Gen-miR-5 derived from Gentianella acuta inhibits PFKP to prevent fibroblast activation and alleviate myocardial fibrosis |