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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated MYH6 Polyclonal antibody

MYH6 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-22281

Synonyms

alpha MHC, ASD3, MYH6, MYHC, MyHC alpha, MYHCA, Myosin 6, Myosin heavy chain 6

Validation Data Gallery

Applications:
  • IF Staining of mouse heart using CL488-22281

    IF Staining of mouse heart using CL488-22281

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488 MYH6 antibody (CL488-22281) at dilution of 1:200.

Tested Applications

Positive IF detected inmouse heart tissue
Planning an IHC experiment? We recommend our IHCeasy MYH6 Ready-To-Use IHC Kit. MYH6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 22281-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-22281 targets MYH6 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name myosin, heavy chain 6, cardiac muscle, alpha
Calculated molecular weight 224 kDa
Observed molecular weight 200-220 kDa
GenBank accession numberNM_002471
Gene symbol MYH6
Gene ID (NCBI) 4624
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYH6 encodes the alpha-cardiac myosin heavy chain (aMHC) which is a major contractile protein exclusively expressed in the myocardium of the heart. Expression of MYH6 is down-regulated in cardiac myopathy and failing hearts. Defects in MYH6 lead to congenital heart disease (CHD), and a spectrum of phenotypes ranging from hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM). (15737621, 20656787)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MYH6 antibody CL488-22281Download protocol
Standard Protocols
Click here to view our Standard Protocols