Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HEK-293T cells, mouse cerebellum tissue, NIH/3T3 cells, mouse brain tissue, rat brain tissue, rat cerebellum tissue |
| Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse cerebellum tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86157-3-RR targets MYH10 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | MYH10 fusion protein Ag16113 相同性解析による交差性が予測される生物種 |
| Full Name | myosin, heavy chain 10, non-muscle |
| Calculated molecular weight | 1985 aa, 230 kDa |
| Observed molecular weight | 229 kDa |
| GenBank accession number | BC150634 |
| Gene Symbol | MYH10 |
| Gene ID (NCBI) | 4628 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P35580 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MYH10 (non-muscle II-b, NM IIB) is a member of non-muscle myosin II which plays fundamental roles in the maintenance of cell morphology, cell adhesion, and migration, as well as cell division. MYH10 is mainly present in nerve cells, megakaryocytes, and other non-muscle cells. It has been reported to mediate centrosome reorientation during cell migration and contribute to ciliogenesis. Overexpression of MYH10 has been observed in breast cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MYH10 antibody 86157-3-RR | Download protocol |
| IHC protocol for MYH10 antibody 86157-3-RR | Download protocol |
| IF protocol for MYH10 antibody 86157-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |