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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYH10 Recombinant antibody (86157-3-RR)

    WB analysis using 86157-3-RR (same clone as 86157-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86157-3-RR (MYH10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86157-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MYH10 Recombinant antibody (86157-3-RR)

    WB analysis using 86157-3-RR (same clone as 86157-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86157-3-RR (MYH10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86157-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using MYH10 Recombinant antibody (86157-3-RR)

    IHC staining of mouse cerebellum using 86157-3-RR (same clone as 86157-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 86157-3-RR (MYH10 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86157-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using MYH10 Recombinant antibody (86157-3-RR)

    IHC staining of mouse cerebellum using 86157-3-RR (same clone as 86157-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 86157-3-RR (MYH10 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86157-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using MYH10 Recombinant antibody (86157-3-RR)

    IF Staining of mouse cerebellum using 86157-3-RR (same clone as 86157-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using MYH10 antibody (86157-3-RR, Clone: 250791D5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86157-3-PBS in a different storage buffer formulation.

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MYH10 Recombinant antibody, PBS Only

MYH10 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 86157-3-PBS
Clone No.250791D5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Myosin 10, Myosin heavy chain 10, Myosin heavy chain, non-muscle IIb, Myosin-10, NMMHC II-b

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86157-3-PBS targets MYH10 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MYH10 fusion protein Ag16113 相同性解析による交差性が予測される生物種
Full Name myosin, heavy chain 10, non-muscle
Calculated molecular weight 1985 aa, 230 kDa
Observed molecular weight 229 kDa
GenBank accession numberBC150634
Gene Symbol MYH10
Gene ID (NCBI) 4628
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35580
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MYH10 (non-muscle II-b, NM IIB) is a member of non-muscle myosin II which plays fundamental roles in the maintenance of cell morphology, cell adhesion, and migration, as well as cell division. MYH10 is mainly present in nerve cells, megakaryocytes, and other non-muscle cells. It has been reported to mediate centrosome reorientation during cell migration and contribute to ciliogenesis. Overexpression of MYH10 has been observed in breast cancer.