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Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using MYH1-motor domain Polyclonal antibody (22282-1-AP)

    WB analysis of human skeletal muscle using 22282-1-AP (same clone as 22282-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22282-1-AP (MYH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22282-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using MYH1-motor domain Polyclonal antibody (22282-1-AP)

    IHC staining of human skeletal muscle using 22282-1-AP (same clone as 22282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22282-1-AP (MYH1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22282-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using MYH1-motor domain Polyclonal antibody (22282-1-AP)

    IHC staining of human skeletal muscle using 22282-1-AP (same clone as 22282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22282-1-AP (MYH1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22282-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of C2C12 cells using MYH1-motor domain Polyclonal antibody (22282-1-AP)

    FC experiment of C2C12 using 22282-1-AP (same clone as 22282-1-PBS)

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human MYH1-motor domain (22282-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 22282-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22282-1-PBS targets MYH1-motor domain in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name myosin, heavy chain 1, skeletal muscle, adult
Calculated molecular weight 223 kDa
Observed molecular weight 223 kDa
GenBank accession numberNM_005963
Gene Symbol MYH1
Gene ID (NCBI) 4619
RRIDAB_2879059
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP12882
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.