Validation Data Gallery
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Tested Applications
| Positive WB detected in | human skeletal muscle tissue |
| Positive IHC detected in | human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22282-1-AP targets MYH1-motor domain in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | myosin, heavy chain 1, skeletal muscle, adult |
| Calculated molecular weight | 223 kDa |
| Observed molecular weight | 223 kDa |
| GenBank accession number | NM_005963 |
| Gene Symbol | MYH1 |
| Gene ID (NCBI) | 4619 |
| RRID | AB_2879059 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P12882 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for MYH1-motor domain antibody 22282-1-AP | Download protocol |
| IHC protocol for MYH1-motor domain antibody 22282-1-AP | Download protocol |
| WB protocol for MYH1-motor domain antibody 22282-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
