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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MX2 Polyclonal antibody (13278-1-AP)

    WB analysis using 13278-1-AP (same clone as 13278-1-PBS)

    Untreated and IFN beta treated THP-1 cell lysates were subjected to SDS PAGE followed by western blot with 13278-1-AP (MX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13278-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using MX2 Polyclonal antibody (13278-1-AP)

    IHC staining of human stomach using 13278-1-AP (same clone as 13278-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13278-1-AP (MX2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13278-1-PBS in a different storage buffer formulation.

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MX2 Polyclonal antibody, PBS Only

MX2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 13278-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

p78-related protein, p78 related protein, Myxovirus resistance protein 2, MXB, Interferon-regulated resistance GTP-binding protein MxB

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13278-1-PBS targets MX2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MX2 fusion protein Ag4020 相同性解析による交差性が予測される生物種
Full Name myxovirus (influenza virus) resistance 2 (mouse)
Calculated molecular weight 715 aa, 82 kDa
Observed molecular weight 70-82 kDa
GenBank accession numberBC035293
Gene Symbol MX2
Gene ID (NCBI) 4600
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20592
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Myxovirus resistance 2 (MX2, also known as MxB) is a member of both the dynamin family and the family of large GTPases. MX2 is an interferon-stimulated gene that inhibits the nuclear import of HIV-1 and interacts with the viral capsid and cellular nuclear transport machinery. MX2 mediates the effects of IFN signaling in a cell type and context-dependent manner that can influence cells sensitivity to MAPK pathway targeted therapies. MX2 is also associated with the tumorigenesis process in melanoma(PMID: 33734579).