Validation Data Gallery
Tested Applications
| Positive WB detected in | THP-1 cells, IFN beta treated THP-1 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 1 publications below |
Product Information
13278-1-AP targets MX2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4020 Product name: Recombinant human MX2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 369-715 aa of BC035293 Sequence: RLAERLTTELIMHIQKSLPLLEGQIRESHQKATEELRRCGADIPSQEADKMFFLIEKIKMFNQDIEKLVEGEEVVRENETRLYNKIREDFKNWVGILATNTQKVKNIIHEEVEKYEKQYRGKELLGFVNYKTFEIIVHQYIQQLVEPALSMLQKAMEIIQQAFINVAKKHFGEFFNLNQTVQSTIEDIKVKHTAKAENMIQLQFRMEQMVFCQDQIYSVVLKKVREEIFNPLGTPSQNMKLNSHFPSNESSVSSFTEIGIHLNAYFLETSKRLANQIPFIIQYFMLRENGDSLQKAMMQILQEKNRYSWLLQEQSETATKRRILKERIYRLTQARHALCQFSSKEIH 相同性解析による交差性が予測される生物種 |
| Full Name | myxovirus (influenza virus) resistance 2 (mouse) |
| Calculated molecular weight | 715 aa, 82 kDa |
| Observed molecular weight | 70-82 kDa |
| GenBank accession number | BC035293 |
| Gene Symbol | MX2 |
| Gene ID (NCBI) | 4600 |
| RRID | AB_3669157 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20592 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Myxovirus resistance 2 (MX2, also known as MxB) is a member of both the dynamin family and the family of large GTPases. MX2 is an interferon-stimulated gene that inhibits the nuclear import of HIV-1 and interacts with the viral capsid and cellular nuclear transport machinery. MX2 mediates the effects of IFN signaling in a cell type and context-dependent manner that can influence cells sensitivity to MAPK pathway targeted therapies. MX2 is also associated with the tumorigenesis process in melanoma(PMID: 33734579).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MX2 antibody 13278-1-AP | Download protocol |
| WB protocol for MX2 antibody 13278-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Poxviruses Evade Cytosolic Sensing through Disruption of an mTORC1-mTORC2 Regulatory Circuit. | ||
J Virol mTOR Dysregulation by Vaccinia Virus F17 Controls Multiple Processes with Varying Roles in Infection. | ||
J Virol YTHDF2 Is Downregulated in Response to Host Shutoff Induced by DNA Virus Infection and Regulates Interferon-Stimulated Gene Expression | ||
Nat Commun The poxvirus F17 protein counteracts mitochondrially orchestrated antiviral responses | ||
Ann Med A novel glycogene-related signature for prognostic prediction and immune microenvironment assessment in kidney renal clear cell carcinoma |