Validation Data Gallery

Filter: AllWBIHC

WB analysis using 28703-1-AP (same clone as 28703-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 28703-1-AP (MUC4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28703-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 28703-1-AP (same clone as 28703-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28703-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 28703-1-AP (same clone as 28703-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28703-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 28703-1-AP (same clone as 28703-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28703-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 28703-1-AP (same clone as 28703-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28703-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 28703-1-AP (same clone as 28703-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28703-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 28703-1-AP (same clone as 28703-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28703-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 28703-1-AP (same clone as 28703-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28703-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 28703-1-AP (same clone as 28703-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28703-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 28703-1-AP (same clone as 28703-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28703-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 28703-1-AP (same clone as 28703-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28703-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 28703-1-AP (same clone as 28703-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28703-1-PBS in a different storage buffer formulation.

MUC4 Polyclonal antibody, PBS Only

MUC4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 28703-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Mucin 4, MUC-4, MUC 4, ASGP 2, ASGP 1 Mucin 4 beta chain

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28703-1-PBS targets MUC4 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag29799 Product name: Recombinant human MUC4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 29-225 aa of NM_018406 Sequence: TTEDTLITGSKTAAPVTSTGSTTATLEGQSTAASSRTSNQDISASSQNHQTKSTETTSKAQTDTLTQMMTSTLFSSPSVHNVMETVTQETAPPDEMTTSFPSSVTNTLMMTSKTITMTTSTDSTLGNTEETSTAGTESSTPVTSAVSITAGQEGQSRTTSWRTSIQDTSASSQNHWTRSTQTTRESQTSTLTHRTTS 相同性解析による交差性が予測される生物種
Full Name	mucin 4, cell surface associated
Calculated molecular weight	231 kDa
Observed molecular weight	231 kDa, 113 kDa
GenBank accession number	NM_018406
Gene Symbol	MUC4
Gene ID (NCBI)	4585
RRID	AB_2918192
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q99102
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

MUC4, also named as HSA276359, ASGP, MUC-4, testis mucin and mucin-4 is a high molecular weight transmembrane mucin that is overexpressed in many carcinomas and is a risk factor associated with a poor prognosis. MUC4 have a key role in the progression of pancreatic cancer. MUC4 helps in the docking of tumor cells on the endothelial surface.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 28703-1-AP (same clone as 28703-1-PBS)

IHC staining of human prostate cancer using 28703-1-AP (same clone as 28703-1-PBS)

IHC staining of human prostate cancer using 28703-1-AP (same clone as 28703-1-PBS)

IHC staining of human colon using 28703-1-AP (same clone as 28703-1-PBS)

IHC staining of human breast cancer using 28703-1-AP (same clone as 28703-1-PBS)

IHC staining of human breast cancer using 28703-1-AP (same clone as 28703-1-PBS)

IHC staining of human colon cancer using 28703-1-AP (same clone as 28703-1-PBS)