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Species Reactivity

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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MUC4 Polyclonal antibody (28703-1-AP)

    WB analysis using 28703-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28703-1-AP (MUC4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MUC4 Polyclonal antibody (28703-1-AP)

    IHC staining of human prostate cancer using 28703-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MUC4 Polyclonal antibody (28703-1-AP)

    IHC staining of human prostate cancer using 28703-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using MUC4 Polyclonal antibody (28703-1-AP)

    IHC staining of human colon using 28703-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MUC4 Polyclonal antibody (28703-1-AP)

    IHC staining of human breast cancer using 28703-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MUC4 Polyclonal antibody (28703-1-AP)

    IHC staining of human breast cancer using 28703-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MUC4 Polyclonal antibody (28703-1-AP)

    IHC staining of human colon cancer using 28703-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MUC4 Polyclonal antibody (28703-1-AP)

    IHC staining of human colon cancer using 28703-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MUC4 Polyclonal antibody (28703-1-AP)

    IHC staining of human lung cancer using 28703-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MUC4 Polyclonal antibody (28703-1-AP)

    IHC staining of human lung cancer using 28703-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MUC4 Polyclonal antibody (28703-1-AP)

    IHC staining of human pancreas cancer using 28703-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MUC4 Polyclonal antibody (28703-1-AP)

    IHC staining of human pancreas cancer using 28703-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MUC4 Polyclonal antibody

MUC4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28703-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Mucin 4, MUC-4, MUC 4, ASGP 2, ASGP 1 Mucin 4 beta chain

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inhuman prostate cancer tissue, human breast cancer tissue, human colon tissue, human colon cancer tissue, human lung cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28703-1-AP targets MUC4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag29799

Product name: Recombinant human MUC4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 29-225 aa of NM_018406

Sequence: TTEDTLITGSKTAAPVTSTGSTTATLEGQSTAASSRTSNQDISASSQNHQTKSTETTSKAQTDTLTQMMTSTLFSSPSVHNVMETVTQETAPPDEMTTSFPSSVTNTLMMTSKTITMTTSTDSTLGNTEETSTAGTESSTPVTSAVSITAGQEGQSRTTSWRTSIQDTSASSQNHWTRSTQTTRESQTSTLTHRTTS

相同性解析による交差性が予測される生物種
Full Name mucin 4, cell surface associated
Calculated molecular weight 231 kDa
Observed molecular weight231 kDa, 113 kDa
GenBank accession numberNM_018406
Gene Symbol MUC4
Gene ID (NCBI) 4585
RRIDAB_2918192
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99102
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MUC4, also named as HSA276359, ASGP, MUC-4, testis mucin and mucin-4 is a high molecular weight transmembrane mucin that is overexpressed in many carcinomas and is a risk factor associated with a poor prognosis. MUC4 have a key role in the progression of pancreatic cancer. MUC4 helps in the docking of tumor cells on the endothelial surface.

Protocols

Product Specific Protocols
WB protocol for MUC4 antibody 28703-1-APDownload protocol
IHC protocol for MUC4 antibody 28703-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols