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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon tissue using MUC2 Recombinant monoclonal antibody (82792-2-RR)

    IHC staining of human colon using 82792-2-RR (same clone as 82792-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82792-2-RR (MUC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82792-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using MUC2 Recombinant monoclonal antibody (82792-2-RR)

    IHC staining of human colon using 82792-2-RR (same clone as 82792-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82792-2-RR (MUC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82792-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using MUC2 Recombinant monoclonal antibody (82792-2-RR)

    IHC staining of mouse colon using 82792-2-RR (same clone as 82792-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82792-2-RR (MUC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82792-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using MUC2 Recombinant monoclonal antibody (82792-2-RR)

    IHC staining of mouse colon using 82792-2-RR (same clone as 82792-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82792-2-RR (MUC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82792-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using MUC2 Recombinant monoclonal antibody (82792-2-RR)

    IHC staining of human colon using 82792-2-RR (same clone as 82792-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82792-2-RR (MUC2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82792-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon tissue using MUC2 Recombinant monoclonal antibody (82792-2-RR)

    IF Staining of human colon using 82792-2-RR (same clone as 82792-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using MUC2 antibody (82792-2-RR, Clone: 250669F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82792-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using MUC2 Recombinant monoclonal antibody (82792-2-RR)

    IF Staining of mouse colon using 82792-2-RR (same clone as 82792-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using MUC2 antibody (82792-2-RR, Clone: 250669F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82792-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82792-2-RR

    Affinity and Kinetic Characterization of 82792-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82792-2-RR against Human MUC2 were performed. The affinity constant is below 1 pM.

MUC2 Recombinant monoclonal antibody, PBS Only

MUC2 Uni-rAb® Recombinant Antibody for IHC, IF-P, Indirect ELISA
Cat No. 82792-2-PBS
Clone No.250669F4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, Indirect ELISA

Synonyms

MUC 2, MUC-2, Mucin 2, SMUC

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82792-2-PBS targets MUC2 in IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag25800

Product name: Recombinant human MUC2 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 726-850 aa of M94132

Sequence: MYLEAGDVVVRQEERCVCRDGRLHCRQIRLIGQSCTAPKIHMDCSNLTALATSKPRALSCQTLAAGYYHTECVSGCVCPDGLMDDGRGGCVVEKECPCVHNNDLYSSGAKIKVDCNTCTCKRGRWV

相同性解析による交差性が予測される生物種
Full Name mucin 2, oligomeric mucus/gel-forming
Calculated molecular weight 540 kDa
GenBank accession numberM94132
Gene Symbol MUC2
Gene ID (NCBI) 4583
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. Downregulation of this gene has been observed in patients with Crohn disease and ulcerative colitis.

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