Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-84328 targets MTMR4 in IF/ICC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag4310 Product name: Recombinant human MTMR4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 845-1195 aa of BC035609 Sequence: SHQEQLSSVPDLTHGEEDIGKRGNNRNGQLLENPRFGKMPLELVRKPISQSQISEFSFLGSNWDSFQGMVTSFPSGEATPRRLLSYGCCSKRPNSKQMRATGPCFGGQWAQREGVKSPVCSSHSNGHCTGPGGKNQMWLSSHPKQVSSTKPVPLNCPSPVPPLYLDDDGLPFPTDVIQHRLRQIEAGYKQEVEQLRRQVRELQMRLDIRHCCAPPAEPPMDYEDDFTCLKESDGSDTEDFGSDHSEDCLSEASWEPVDKKETEVTRWVPDHMASHCYNCDCEFWLAKRRHHCRNCGNVFCAGCCHLKLPIPDQQLYDPVLVCNSCYEHIQVSRARELMSQQLKKPIATASS 相同性解析による交差性が予測される生物種 |
| Full Name | myotubularin related protein 4 |
| Calculated molecular weight | 1195 aa, 133 kDa |
| Observed molecular weight | 133 kDa |
| GenBank accession number | BC035609 |
| Gene Symbol | MTMR4 |
| Gene ID (NCBI) | 9110 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NYA4 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Myotubularin-related protein-4 (MTMR4), also named FYVE-DSP2, is a member of the myotubularin family of phosphoinositide phosphatases that selectively degrade the 3-position phosphate from the inositol ring of PtdIns(3)P and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) (PMID: 31543504). MTMR4 knockdown markedly suppressed the motility, fusion, and fission of PI(3)P- enriched structures, resulting in decreases in late endosomes, autophagosomes, and lysosomes, and enlargement of PI(3)P- enriched early and late endosomes(PMID: 29962048).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 MTMR4 antibody CL488-84328 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |