Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, rat testis tissue, Jurkat cells, mouse testis tissue |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86608-3-RR targets MTHFD2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag2911 Product name: Recombinant human MTHFD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC017054 Sequence: MAATSLMSALAARLLQPAHSCSLRLRPFHLAAVRNEAVVISGRKLAQQIKQEVRQEVEEWVASGNKRPHLSVILVGENPASHSYVLNKTRAAAVVGINSETIMKPASISEEELLNLINKLNNDDNVDGLLVQLPLPEHIDERRICNAVSPDKDVDGFHVINVGRMCLDQYSMLPATPWGVWEIIKRTGIPTLGKNVVVAGRSKNVGMPIAMLLHTDGAHERPGGDATVTISHRYTPKEQLKKHTILADIVISAAGIPNLITADMIKEGAAVIDVGINRVHDPVTAKPKLVGDVDFEGVRQKAGYITPVPGGVGPMTVAMLMKNTIIAAKKVLRLEEREVLKSKELGVATN 相同性解析による交差性が予測される生物種 |
| Full Name | methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase |
| Calculated molecular weight | 350 aa, 38 kDa |
| Observed molecular weight | 33-38 kDa |
| GenBank accession number | BC017054 |
| Gene Symbol | MTHFD2 |
| Gene ID (NCBI) | 10797 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P13995 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) is a folate-coupled mitochondrial metabolic enzyme characterized by methylenetetrahydrofolate dehydrogenase and cyclohydrolase activity. It was demonstrated that suppression of MTHFD2 inhibits methylation reactions, decreases protein synthesis and disrupts redox homeostasis, which may ultimately result in significant changes in cellular metabolic phenotype. MTHFD2 is reported to be up‐regulated in various tumours, such as lung adenocarcinoma(PMID: 34121323) and breast cancer(PMID: 34007312).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for MTHFD2 antibody 86608-3-RR | Download protocol |
| IF protocol for MTHFD2 antibody 86608-3-RR | Download protocol |
| WB protocol for MTHFD2 antibody 86608-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |