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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MTHFD2 Recombinant monoclonal antibody (86608-3-RR)

    WB analysis using 86608-3-RR (same clone as 86608-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86608-3-RR (MTHFD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86608-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat testis tissue using MTHFD2 Recombinant monoclonal antibody (86608-3-RR)

    WB analysis of rat testis using 86608-3-RR (same clone as 86608-3-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 86608-3-RR (MTHFD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86608-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MTHFD2 Recombinant monoclonal antibody (86608-3-RR)

    IF Staining of HepG2 using 86608-3-RR (same clone as 86608-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MTHFD2 antibody (86608-3-RR, Clone: 251431F2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86608-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using MTHFD2 Recombinant monoclonal antibody (86608-3-RR)

    FC experiment of A549 using 86608-3-RR (same clone as 86608-3-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug MTHFD2 Recombinant monoclonal antibody (86608-3-RR, Clone:251431F2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 86608-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86608-3-RR

    Affinity and Kinetic Characterization of 86608-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86608-3-RR against Human MTHFD2 were performed. The affinity constant is 2.95 nM.

MTHFD2 Recombinant monoclonal antibody, PBS Only

MTHFD2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 86608-3-PBS
Clone No.251431F2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial, Methenyltetrahydrofolate cyclohydrolase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86608-3-PBS targets MTHFD2 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag2911

Product name: Recombinant human MTHFD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC017054

Sequence: MAATSLMSALAARLLQPAHSCSLRLRPFHLAAVRNEAVVISGRKLAQQIKQEVRQEVEEWVASGNKRPHLSVILVGENPASHSYVLNKTRAAAVVGINSETIMKPASISEEELLNLINKLNNDDNVDGLLVQLPLPEHIDERRICNAVSPDKDVDGFHVINVGRMCLDQYSMLPATPWGVWEIIKRTGIPTLGKNVVVAGRSKNVGMPIAMLLHTDGAHERPGGDATVTISHRYTPKEQLKKHTILADIVISAAGIPNLITADMIKEGAAVIDVGINRVHDPVTAKPKLVGDVDFEGVRQKAGYITPVPGGVGPMTVAMLMKNTIIAAKKVLRLEEREVLKSKELGVATN

相同性解析による交差性が予測される生物種
Full Name methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
Calculated molecular weight 350 aa, 38 kDa
Observed molecular weight33-38 kDa
GenBank accession numberBC017054
Gene Symbol MTHFD2
Gene ID (NCBI) 10797
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP13995
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) is a folate-coupled mitochondrial metabolic enzyme characterized by methylenetetrahydrofolate dehydrogenase and cyclohydrolase activity. It was demonstrated that suppression of MTHFD2 inhibits methylation reactions, decreases protein synthesis and disrupts redox homeostasis, which may ultimately result in significant changes in cellular metabolic phenotype. MTHFD2 is reported to be up‐regulated in various tumours, such as lung adenocarcinoma(PMID: 34121323) and breast cancer(PMID: 34007312).

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