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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MTCO2 Recombinant antibody

MTCO2 Recombinant Antibody for WB, IP, IF, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IP, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

230448C9

Cat no : 83132-7-RR

Synonyms

COII, COX2, COXII, cytochrome c oxidase II, Cytochrome c oxidase subunit 2, MT CO2, MTCO2

Validation Data Gallery

Applications:
  • WB analysis using 83132-7-RR

    WB analysis using 83132-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83132-7-RR (MTCO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 83132-7-RR

    IP experiment of HeLa using 83132-7-RR

    IP result of anti-MTCO2 (IP:83132-7-RR, 4ug; Detection:83132-7-RR 1:3000) with HeLa cells lysate 1750 ug.

  • IF Staining of HepG2 using 83132-7-RR

    IF Staining of HepG2 using 83132-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MTCO2 antibody (83132-7-RR, Clone: 230448C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • FC experiment of HepG2 using 83132-7-RR

    FC experiment of HepG2 using 83132-7-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human MTCO2 (83132-7-RR, Clone:230448C9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, U-251 cells, A549 cells, MDA-MB-231 cells
Positive IP detected inHeLa cells
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MTCO2/COX2 Ready-To-Use IHC Kit. MTCO2/COX2 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

83132-7-RR targets MTCO2 in WB, IP, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name cytochrome c oxidase II
Calculated molecular weight 26 kDa
Observed molecular weight 23-26 kDa
GenBank accession numberYP_003024029
Gene symbol COX2
Gene ID (NCBI) 4513
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MTCO2, also named as COII, COXII, and COX2, belongs to the cytochrome c oxidase subunit 2 family. It is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Defects in COX2 are a cause of mitochondrial complex IV deficiency (MT-C4D).

Protocols

Product Specific Protocols
WB protocol for MTCO2 antibody 83132-7-RRDownload protocol
IF protocol for MTCO2 antibody 83132-7-RRDownload protocol
IP protocol for MTCO2 antibody 83132-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols