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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MTCO2 Recombinant monoclonal antibody (83132-7-RR)

    WB analysis using 83132-7-RR (same clone as 83132-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83132-7-RR (MTCO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83132-7-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MTCO2 Recombinant monoclonal antibody (83132-7-RR)

    WB analysis using 83132-7-RR (same clone as 83132-7-PBS)

    WB result of MTCO2 antibody (MTCO2; 1:3000; incubated at room temperature for 1.5 hours) with 143B and 143B.TK.ρ0 cell. 143B is wild-type cell line, 143B.TK.ρ0 is mtDNA deletion cell line. This data was developed using the same antibody clone with 83132-7-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using MTCO2 Recombinant monoclonal antibody (83132-7-RR)

    IP experiment of HeLa using 83132-7-RR (same clone as 83132-7-PBS)

    IP result of anti-MTCO2 (IP:83132-7-RR, 4ug; Detection:83132-7-RR 1:3000) with HeLa cells lysate 1750 ug. This data was developed using the same antibody clone with 83132-7-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using MTCO2 Recombinant monoclonal antibody (83132-7-RR)

    IHC staining of human ovarian cancer using 83132-7-RR (same clone as 83132-7-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83132-7-RR (MTCO2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83132-7-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using MTCO2 Recombinant monoclonal antibody (83132-7-RR)

    IHC staining of human stomach cancer using 83132-7-RR (same clone as 83132-7-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83132-7-RR (MTCO2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83132-7-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MTCO2 Recombinant monoclonal antibody (83132-7-RR)

    IF Staining of HepG2 using 83132-7-RR (same clone as 83132-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MTCO2 antibody (83132-7-RR, Clone: 230448C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83132-7-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using MTCO2 Recombinant monoclonal antibody (83132-7-RR)

    FC experiment of HeLa using 83132-7-RR (same clone as 83132-7-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human MTCO2 (83132-7-RR, Clone:230448C9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83132-7-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MTCO2 Recombinant monoclonal antibody, PBS Only

MTCO2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 83132-7-PBS
Clone No.230448C9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

COX2, 230448C9, COII, COX 2, COXII

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83132-7-PBS targets MTCO2 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name cytochrome c oxidase II
Calculated molecular weight 26 kDa
Observed molecular weight 23-26 kDa
GenBank accession numberYP_003024029
Gene Symbol MTCO2
Gene ID (NCBI) 4513
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP00403
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MTCO2, also named as COII, COXII, and COX2, belongs to the cytochrome c oxidase subunit 2 family. It is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Defects in COX2 are a cause of mitochondrial complex IV deficiency (MT-C4D).