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Validation Data Gallery

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  • Western Blot (WB) analysis of U2OS cells using MTBP Polyclonal antibody (28356-1-AP)

    WB analysis of U2OS using 28356-1-AP (same clone as 28356-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 28356-1-AP (MTBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28356-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MTBP Polyclonal antibody (28356-1-AP)

    WB analysis using 28356-1-AP (same clone as 28356-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28356-1-AP (MTBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28356-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MTBP Polyclonal antibody (28356-1-AP)

    IHC staining of human lung cancer using 28356-1-AP (same clone as 28356-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28356-1-AP (MTBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28356-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MTBP Polyclonal antibody (28356-1-AP)

    IHC staining of human lung cancer using 28356-1-AP (same clone as 28356-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28356-1-AP (MTBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28356-1-PBS in a different storage buffer formulation.

MTBP Polyclonal antibody, PBS Only

MTBP Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 28356-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28356-1-PBS targets MTBP in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag28393

Product name: Recombinant human MTBP protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 98-253 aa of BC013136

Sequence: QEIHFDTEKDKIEDVLQTNIEECLGAVECFEEEDSNSRESLSLADLYEEAAENLHQLSDKLPAPGRAMVDIILLLSDKDPPKLKDYLPTVGALKHLREWYSAKITIAGNHCEINCQKIAEYLSANVVSLEDLRNVIDSKELWRGKIQIWERKFGFE

相同性解析による交差性が予測される生物種
Full Name Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse) binding protein, 104kDa
Calculated molecular weight 102 kDa
Observed molecular weight 38 kDa, 110 kDa
GenBank accession numberBC013136
Gene Symbol MTBP
Gene ID (NCBI) 27085
RRIDAB_2881118
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96DY7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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