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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MTBP Polyclonal antibody

MTBP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28356-1-AP

Synonyms

hMTBP, Mdm2 binding protein, MDM2BP, MTBP

Validation Data Gallery

Applications:
  • WB analysis of U2OS using 28356-1-AP

    WB analysis of U2OS using 28356-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 28356-1-AP (MTBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 28356-1-AP

    WB analysis using 28356-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28356-1-AP (MTBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 28356-1-AP

    IHC staining of human lung cancer using 28356-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28356-1-AP (MTBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 28356-1-AP

    IHC staining of human lung cancer using 28356-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28356-1-AP (MTBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inU2OS cells, K-562 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

28356-1-AP targets MTBP in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTBP fusion protein Ag28393 相同性解析による交差性が予測される生物種
Full Name Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse) binding protein, 104kDa
Calculated molecular weight 102 kDa
Observed molecular weight 38 kDa, 110 kDa
GenBank accession numberBC013136
Gene symbol MTBP
Gene ID (NCBI) 27085
RRIDAB_2881118
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MTBP antibody 28356-1-APDownload protocol
IHC protocol for MTBP antibody 28356-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols