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Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using MRPL49 Polyclonal antibody (15542-1-AP)

    WB analysis of BxPC-3 using 15542-1-AP (same clone as 15542-1-PBS)

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15542-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MRPL49 Polyclonal antibody (15542-1-AP)

    WB analysis of HeLa using 15542-1-AP (same clone as 15542-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15542-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using MRPL49 Polyclonal antibody (15542-1-AP)

    WB analysis of A549 using 15542-1-AP (same clone as 15542-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15542-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MRPL49 Polyclonal antibody (15542-1-AP)

    WB analysis of HeLa using 15542-1-AP (same clone as 15542-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15542-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using MRPL49 Polyclonal antibody (15542-1-AP)

    IP experiment of HeLa using 15542-1-AP (same clone as 15542-1-PBS)

    IP result of anti-MRPL49 (IP:15542-1-AP, 4ug; Detection:15542-1-AP 1:500) with HeLa cells lysate 3360ug. This data was developed using the same antibody clone with 15542-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MRPL49 Polyclonal antibody (15542-1-AP)

    IHC staining of human breast cancer using 15542-1-AP (same clone as 15542-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15542-1-AP (MRPL49 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15542-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MRPL49 Polyclonal antibody (15542-1-AP)

    IHC staining of human breast cancer using 15542-1-AP (same clone as 15542-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15542-1-AP (MRPL49 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15542-1-PBS in a different storage buffer formulation.

MRPL49 Polyclonal antibody, PBS Only

MRPL49 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 15542-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

C11orf4, Large ribosomal subunit protein mL49, MRP-L49, NOF, NOF1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15542-1-PBS targets MRPL49 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7898

Product name: Recombinant human MRPL49 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-166 aa of BC004378

Sequence: MAATMFRATLRGWRTGVQRGCGLRLLSQTQGPPDYPRFVESVDEYQFVERLLPATRIPDPPKHEHYPTPSGWQPPRDPPPNLPYFVRRSRMHNIPVYKDITHGNRQMTVIRKVEGDIWALQKDVEDFLSPLLGKTPVTQVNEVTGTLRIKGYFDQELKAWLLEKGF

相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L49
Calculated molecular weight 19 kDa
Observed molecular weight 19 kDa
GenBank accession numberBC004378
Gene Symbol MRPL49
Gene ID (NCBI) 740
RRIDAB_2146189
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13405
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MRPL49, also named as C11orf4, NOF1, or SW-cl.67, is a 166 amino acid protein, which belongs to the ribosomal protein L49em family. MRPL49 localizes in the Mitochondrion and is widely expressed in various tissues. MRPL49 is a regulator of C3 convertase of classical pathway.

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