Validation Data Gallery
Tested Applications
| Positive WB detected in | BxPC-3 cells, A549 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
Product Information
15542-1-AP targets MRPL49 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7898 Product name: Recombinant human MRPL49 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-166 aa of BC004378 Sequence: MAATMFRATLRGWRTGVQRGCGLRLLSQTQGPPDYPRFVESVDEYQFVERLLPATRIPDPPKHEHYPTPSGWQPPRDPPPNLPYFVRRSRMHNIPVYKDITHGNRQMTVIRKVEGDIWALQKDVEDFLSPLLGKTPVTQVNEVTGTLRIKGYFDQELKAWLLEKGF 相同性解析による交差性が予測される生物種 |
| Full Name | mitochondrial ribosomal protein L49 |
| Calculated molecular weight | 19 kDa |
| Observed molecular weight | 19 kDa |
| GenBank accession number | BC004378 |
| Gene Symbol | MRPL49 |
| Gene ID (NCBI) | 740 |
| RRID | AB_2146189 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13405 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MRPL49, also named as C11orf4, NOF1, or SW-cl.67, is a 166 amino acid protein, which belongs to the ribosomal protein L49em family. MRPL49 localizes in the Mitochondrion and is widely expressed in various tissues. MRPL49 is a regulator of C3 convertase of classical pathway.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MRPL49 antibody 15542-1-AP | Download protocol |
| IHC protocol for MRPL49 antibody 15542-1-AP | Download protocol |
| IP protocol for MRPL49 antibody 15542-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res C7orf30 specifically associates with the large subunit of the mitochondrial ribosome and is involved in translation. | ||
Nucleic Acids Res Replication factors transiently associate with mtDNA at the mitochondrial inner membrane to facilitate replication. | ||
Sci Rep Human Mitochondrial DNA-Protein Complexes Attach to a Cholesterol-Rich Membrane Structure. | ||
Cell Rep MitoRibo-Tag Mice Provide a Tool for In Vivo Studies of Mitoribosome Composition. | ||
Nucleic Acids Res Mitochondrial RNA granules are critically dependent on mtDNA replication factors Twinkle and mtSSB. |