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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPL35 Polyclonal antibody (27339-1-AP)

    WB analysis using 27339-1-AP (same clone as 27339-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27339-1-AP (MRPL35 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27339-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MRPL35 Polyclonal antibody (27339-1-AP)

    IF Staining of HepG2 using 27339-1-AP (same clone as 27339-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL35 antibody (27339-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 27339-1-PBS in a different storage buffer formulation.

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MRPL35 Polyclonal antibody, PBS Only

MRPL35 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 27339-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

MRPL 35, MRP L35, L35mt

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27339-1-PBS targets MRPL35 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPL35 fusion protein Ag26300 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L35
Calculated molecular weight 170 aa, 19 kDa
Observed molecular weight24 kDa
GenBank accession numberBC020651
Gene Symbol MRPL35
Gene ID (NCBI) 51318
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZE8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Mitochondrial ribosomal protein L35 (MRPL35) is a member of the large subunit family of mitochondrial ribosomal proteins. It plays a key role in the assembly of cytochrome C oxidase, involved in the translation of mitochondrial proteins.