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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPL35 Polyclonal antibody (27339-1-AP)

    WB analysis using 27339-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27339-1-AP (MRPL35 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using MRPL35 Polyclonal antibody (27339-1-AP)

    IHC staining of mouse liver using 27339-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27339-1-AP (MRPL35 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using MRPL35 Polyclonal antibody (27339-1-AP)

    IHC staining of mouse liver using 27339-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27339-1-AP (MRPL35 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MRPL35 Polyclonal antibody (27339-1-AP)

    IF Staining of HepG2 using 27339-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL35 antibody (27339-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

MRPL35 Polyclonal antibody

MRPL35 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 27339-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

MRPL 35, MRP L35, L35mt

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse heart tissue, mouse liver tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27339-1-AP targets MRPL35 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26300

Product name: Recombinant human MRPL35 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 61-170 aa of BC020651

Sequence: TTSERNLTCGHTSVILNRMAPVLPSVLKLPVRSLTYFSARKGKRKTVKAVIDRFLRLHCGLWVRRKAGYKKKLWKKTPARKKRLREFVFCNKTQSKLLDKMTTSFWKRRN

相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L35
Calculated molecular weight 170 aa, 19 kDa
Observed molecular weight24 kDa
GenBank accession numberBC020651
Gene Symbol MRPL35
Gene ID (NCBI) 51318
RRIDAB_3669593
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZE8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mitochondrial ribosomal protein L35 (MRPL35) is a member of the large subunit family of mitochondrial ribosomal proteins. It plays a key role in the assembly of cytochrome C oxidase, involved in the translation of mitochondrial proteins.

Protocols

Product Specific Protocols
IF protocol for MRPL35 antibody 27339-1-APDownload protocol
IHC protocol for MRPL35 antibody 27339-1-APDownload protocol
WB protocol for MRPL35 antibody 27339-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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