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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPL35 Polyclonal antibody (27339-1-AP)

    WB analysis using 27339-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27339-1-AP (MRPL35 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MRPL35 Polyclonal antibody (27339-1-AP)

    IF Staining of HepG2 using 27339-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL35 antibody (27339-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

MRPL35 Polyclonal antibody

MRPL35 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 27339-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

MRPL 35, MRP L35, L35mt

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse heart tissue, mouse liver tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27339-1-AP targets MRPL35 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPL35 fusion protein Ag26300 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L35
Calculated molecular weight 170 aa, 19 kDa
Observed molecular weight24 kDa
GenBank accession numberBC020651
Gene Symbol MRPL35
Gene ID (NCBI) 51318
RRIDAB_3669593
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZE8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mitochondrial ribosomal protein L35 (MRPL35) is a member of the large subunit family of mitochondrial ribosomal proteins. It plays a key role in the assembly of cytochrome C oxidase, involved in the translation of mitochondrial proteins.

Protocols

Product Specific Protocols
WB protocol for MRPL35 antibody 27339-1-APDownload protocol
IF protocol for MRPL35 antibody 27339-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols