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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPL20 Polyclonal antibody (16969-1-AP)

    WB analysis using 16969-1-AP (same clone as 16969-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16969-1-AP (MRPL20 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16969-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MRPL20 Polyclonal antibody (16969-1-AP)

    WB analysis using 16969-1-AP (same clone as 16969-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16969-1-AP (MRPL20 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16969-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MRPL20 Polyclonal antibody (16969-1-AP)

    IF Staining of HepG2 using 16969-1-AP (same clone as 16969-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL20 antibody (16969-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 16969-1-PBS in a different storage buffer formulation.

MRPL20 Polyclonal antibody, PBS Only

MRPL20 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 16969-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

L20mt, Large ribosomal subunit protein bL20m, MRP L20, MRP-L20

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16969-1-PBS targets MRPL20 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9160

Product name: Recombinant human MRPL20 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-149 aa of BC009515

Sequence: MVFLTAQLWLRNRVTDRYFRIQEVLKHARHFRGRKNRCYRLAVRTVIRAFVKCTKARYLKKKNMRTLWINRITAASQEHGLKYPALIGNLVKCQVELNRKVLADLAIYEPKTFKSLAALASRRRHEGFAAALGDGKEPEGIFSRVVQYH

相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L20
Calculated molecular weight 149 aa, 17 kDa
Observed molecular weight17 kDa
GenBank accession numberBC009515
Gene Symbol MRPL20
Gene ID (NCBI) 55052
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYC9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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