Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, mouse heart tissue, HeLa cells, HepG2 cells, mouse liver tissue, rat heart tissue, rat liver tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
16969-1-AP targets MRPL20 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9160 Product name: Recombinant human MRPL20 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-149 aa of BC009515 Sequence: MVFLTAQLWLRNRVTDRYFRIQEVLKHARHFRGRKNRCYRLAVRTVIRAFVKCTKARYLKKKNMRTLWINRITAASQEHGLKYPALIGNLVKCQVELNRKVLADLAIYEPKTFKSLAALASRRRHEGFAAALGDGKEPEGIFSRVVQYH 相同性解析による交差性が予測される生物種 |
| Full Name | mitochondrial ribosomal protein L20 |
| Calculated molecular weight | 149 aa, 17 kDa |
| Observed molecular weight | 17 kDa |
| GenBank accession number | BC009515 |
| Gene Symbol | MRPL20 |
| Gene ID (NCBI) | 55052 |
| RRID | AB_3741901 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BYC9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MRPL20 (mitochondrial ribosomal protein L20) is a 149-amino-acid nuclear-encoded protein that belongs to the bacterial ribosomal protein bL20 family. As a structural constituent of the mitochondrial ribosome , MRPL20 localizes to the 39S large subunit, where it integrates into the mitochondrial inner membrane and facilitates the synthesis of mitochondrial genome-encoded proteins-including essential transmembrane subunits of the mitochondrial respiratory chain-by participating in all three phases of mitochondrial translation: initiation, elongation, and termination.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MRPL20 antibody 16969-1-AP | Download protocol |
| WB protocol for MRPL20 antibody 16969-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|