- Featured Product
- KD/KO Validated
CoraLite® Plus 555-conjugated MRPL12 Polyclonal antibody
MRPL12 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 555 Fluorescent Dye
Cat no : CL555-14795
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL555-14795 targets MRPL12 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MRPL12 fusion protein Ag6504 相同性解析による交差性が予測される生物種 |
| Full Name | mitochondrial ribosomal protein L12 |
| Calculated molecular weight | 21 kDa |
| Observed molecular weight | 21 kDa |
| GenBank accession number | BC002344 |
| Gene symbol | MRPL12 |
| Gene ID (NCBI) | 6182 |
| Conjugate | CoraLite® Plus 555 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 554 nm / 570 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Mammalian MRPL12 was the first mitochondrial ribosomal protein to be characterized, and is encoded by a nuclear gene. The protein forms a homodimer, localizes predominantly to mitochondria and binds to the large mitochondrial ribosomal subunit [PMID:862670]. It is required for CycD/Cdk4-induced cell growth. In addition, forming a ribosomal stalk with MRPL7, MRPL12 is required for the recruitment of translation elongation factors Tu and G to the large ribosomal subunit [PMID:12370014]. It also a part of a novel regulatory mechanism that coordinates mitochondrial transcription with translation and/or ribosome biogenesis during human mitochondrial gene expression. The antibody reacts specifically with 21kDa MRPL12 protein.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 555 MRPL12 antibody CL555-14795 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
