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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using MRGPRD Polyclonal antibody (23640-1-AP)

    IHC staining of mouse small intestine using 23640-1-AP (same clone as 23640-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 23640-1-AP (MRGPRD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23640-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat dorsal root ganglion tissue using MRGPRD Polyclonal antibody (23640-1-AP)

    IHC staining of rat dorsal root ganglion using 23640-1-AP (same clone as 23640-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 23640-1-AP (MRGPRD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23640-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using MRGPRD Polyclonal antibody (23640-1-AP)

    IF Staining of mouse small intestine using 23640-1-AP (same clone as 23640-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using MRGPRD antibody (23640-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23640-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using MRGPRD Polyclonal antibody (23640-1-AP)

    IF Staining of mouse small intestine using 23640-1-AP (same clone as 23640-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using MRGPRD antibody (23640-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23640-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23640-1-PBS targets MRGPRD in IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRGPRD fusion protein Ag20316 相同性解析による交差性が予測される生物種
Full Name MAS-related GPR, member D
Calculated molecular weight 321 aa, 36 kDa
GenBank accession numberBC156660
Gene Symbol MRGPRD
Gene ID (NCBI) 116512
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ8TDS7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MRGPRD (MAS-related G-protein coupled receptor, D), also referred to as hGPCR45 or TGR7, was identified as a novel GPCR in murine and human genomes. As for MRGD, its expression was found in dorsal root ganglia (DRG) and co-localized with Vanilloid receptor-1 (VR-1), which is an essential receptor for heat and pain sensation (PMID: 22715397).