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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using MRGPRD Polyclonal antibody (23640-1-AP)

    IHC staining of mouse small intestine using 23640-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 23640-1-AP (MRGPRD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat dorsal root ganglion tissue using MRGPRD Polyclonal antibody (23640-1-AP)

    IHC staining of rat dorsal root ganglion using 23640-1-AP

    Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 23640-1-AP (MRGPRD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using MRGPRD Polyclonal antibody (23640-1-AP)

    IF Staining of mouse small intestine using 23640-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using MRGPRD antibody (23640-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using MRGPRD Polyclonal antibody (23640-1-AP)

    IF Staining of mouse small intestine using 23640-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using MRGPRD antibody (23640-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MRGPRD Polyclonal antibody

MRGPRD Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 23640-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF-P, ELISA

Synonyms

Beta alanine receptor, Beta-alanine receptor, G-protein coupled receptor TGR7, MAS related GPR, member D, Mas-related G-protein coupled receptor member D

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse small intestine tissue, rat dorsal root ganglion tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse small intestine tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23640-1-AP targets MRGPRD in IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20316

Product name: Recombinant human MRGPRD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 230-321 aa of BC156660

Sequence: FLICSLPLSIYWFVLYWLSLPPEMQVLCFSLSRLSSSVSSSANPVIYFLVGSRRSHRLPTRSLGTVLQQALREEPELEGGETPTVGTNEMGA

相同性解析による交差性が予測される生物種
Full Name MAS-related GPR, member D
Calculated molecular weight 321 aa, 36 kDa
GenBank accession numberBC156660
Gene Symbol MRGPRD
Gene ID (NCBI) 116512
RRIDAB_3669414
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ8TDS7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MRGPRD (MAS-related G-protein coupled receptor, D), also referred to as hGPCR45 or TGR7, was identified as a novel GPCR in murine and human genomes. As for MRGD, its expression was found in dorsal root ganglia (DRG) and co-localized with Vanilloid receptor-1 (VR-1), which is an essential receptor for heat and pain sensation (PMID: 22715397).

Protocols

Product Specific Protocols
IF protocol for MRGPRD antibody 23640-1-APDownload protocol
IHC protocol for MRGPRD antibody 23640-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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