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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MLX Monoclonal antibody (67042-1-Ig)

    WB analysis using 67042-1-Ig (same clone as 67042-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67042-1-Ig (MLX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67042-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MLX Monoclonal antibody (67042-1-Ig)

    IHC staining of human breast cancer using 67042-1-Ig (same clone as 67042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67042-1-Ig (MLX antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67042-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MLX Monoclonal antibody (67042-1-Ig)

    IHC staining of human liver cancer using 67042-1-Ig (same clone as 67042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67042-1-Ig (MLX antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67042-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MLX Monoclonal antibody (67042-1-Ig)

    IHC staining of human liver cancer using 67042-1-Ig (same clone as 67042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67042-1-Ig (MLX antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67042-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MLX Monoclonal antibody (67042-1-Ig)

    IHC staining of human breast cancer using 67042-1-Ig (same clone as 67042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67042-1-Ig (MLX antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67042-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MLX Monoclonal antibody (67042-1-Ig)

    IF Staining of HepG2 using 67042-1-Ig (same clone as 67042-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MLX antibody (67042-1-Ig, Clone: 2A8D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67042-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MLX Monoclonal antibody (67042-1-Ig)

    IF Staining of HepG2 using 67042-1-Ig (same clone as 67042-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MLX antibody (67042-1-Ig, Clone: 2A8D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67042-1-PBS in a different storage buffer formulation.

MLX Monoclonal antibody, PBS Only

MLX Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67042-1-PBS
Clone No.2A8D2

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

bHLHd13, MAD7, Max like bHLHZip protein, MAX like protein X, MLX, MXD7, Protein BigMax, TCFL4

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67042-1-PBS targets MLX in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag28729

Product name: Recombinant human MLX protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-244 aa of BC010689

Sequence: MTEPGASPEDPWVKVEYAYSDNSLDPGLFVESTRKGSVVSRANSIGSTSASSVPNTDDEDSDYHQEAYKESYKDRRRRAHTQAEQKRRDAIKRGYDDLQTIVPTCQQQDFSIGSQKLSKAIVLQKTIDYIQFLHKEKKKQEEEVSTLRKDVTALKIMKVNYEQIVKAHQDNPHEGEDQVSDQVKFNVFQGIMDSLFQSFNASISVASFQELSACVFSWIEEHCKPQTLREIVIGVLHQLKNQLY

相同性解析による交差性が予測される生物種
Full Name MAX-like protein X
Calculated molecular weight 298 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC010689
Gene Symbol MLX
Gene ID (NCBI) 6945
RRIDAB_2882356
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9UH92
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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