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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MLX Monoclonal antibody (67042-1-Ig)

    WB analysis using 67042-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67042-1-Ig (MLX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MLX Monoclonal antibody (67042-1-Ig)

    IHC staining of human breast cancer using 67042-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67042-1-Ig (MLX antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MLX Monoclonal antibody (67042-1-Ig)

    IHC staining of human liver cancer using 67042-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67042-1-Ig (MLX antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MLX Monoclonal antibody (67042-1-Ig)

    IHC staining of human liver cancer using 67042-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67042-1-Ig (MLX antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MLX Monoclonal antibody (67042-1-Ig)

    IHC staining of human breast cancer using 67042-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67042-1-Ig (MLX antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MLX Monoclonal antibody (67042-1-Ig)

    IF Staining of HepG2 using 67042-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MLX antibody (67042-1-Ig, Clone: 2A8D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). DAPI (blue).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MLX Monoclonal antibody (67042-1-Ig)

    IF Staining of HepG2 using 67042-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MLX antibody (67042-1-Ig, Clone: 2A8D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

MLX Monoclonal antibody

MLX Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67042-1-Ig
Clone No.2A8D2

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

bHLHd13, MAD7, Max like bHLHZip protein, MAX like protein X, MLX, MXD7, Protein BigMax, TCFL4

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67042-1-Ig targets MLX in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag28729

Product name: Recombinant human MLX protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-244 aa of BC010689

Sequence: MTEPGASPEDPWVKVEYAYSDNSLDPGLFVESTRKGSVVSRANSIGSTSASSVPNTDDEDSDYHQEAYKESYKDRRRRAHTQAEQKRRDAIKRGYDDLQTIVPTCQQQDFSIGSQKLSKAIVLQKTIDYIQFLHKEKKKQEEEVSTLRKDVTALKIMKVNYEQIVKAHQDNPHEGEDQVSDQVKFNVFQGIMDSLFQSFNASISVASFQELSACVFSWIEEHCKPQTLREIVIGVLHQLKNQLY

相同性解析による交差性が予測される生物種
Full Name MAX-like protein X
Calculated molecular weight 298 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC010689
Gene Symbol MLX
Gene ID (NCBI) 6945
RRIDAB_2882356
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9UH92
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for MLX antibody 67042-1-IgDownload protocol
IHC protocol for MLX antibody 67042-1-IgDownload protocol
WB protocol for MLX antibody 67042-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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