Validation Data Gallery
Product Information
ChromoTek’s MK2-Trap Agarose are affinity beads for IP of MK2-fusion proteins. It comprises a MK2 Nanobody/ VHH conjugated to agarose beads.
| Description | Immunoprecipitation of MK2 with MK2-Trap Agarose.
• Fast, reliable & efficient one-step immunoprecipitation • Short incubation time (1 h at 4°C) • No heavy & light antibody chains • Stable under harsh washing conditions • Suitable for downstream mass spec analysis |
| Applications | IP, CoIP |
| Specificity/Target | MK2 (MAP kinase-activated protein kinase 2, MAPKAP-K2) |
| Tested Reactivity | Human, Mouse, Hamster Posttranslational modifications: MK2-Trap recognizes unphosphorylated MK2 and Phospho-MK2 (Thr222). Specificity on Phospho-MK2 (Thr334) was not tested. |
| Conjugate | Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) |
| Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Compatibility with mass spectrometry | The MK2-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2631376 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Upon receipt store at +4°C. Do not freeze! |
| Size | 25ul/reactions (eg：20rxns=500ul slurry) |
Publications
| Application | Title |
|---|---|
Proc Natl Acad Sci U S A MK2 degradation as a sensor of signal intensity that controls stress-induced cell fate. | |
Cell Rep Alternative Translation Initiation Generates a Functionally Distinct Isoform of the Stress-Activated Protein Kinase MK2. |