• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MITF Recombinant antibody (83803-2-RR)

    IF Staining of HepG2 using 83803-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MITF antibody (83803-2-RR, Clone: 240815E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using MITF Recombinant antibody (83803-2-RR)

    IF Staining of A431 using 83803-2-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using MITF antibody (83803-2-RR, Clone: 240815E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MITF Recombinant monoclonal antibody (83803-2-RR)

    IF Staining of HeLa using 83803-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MITF antibody (83803-2-RR, Clone: 240815E7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using MITF Recombinant antibody (83803-2-RR)

    FC experiment of HeLa using 83803-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MITF Recombinant antibody (83803-2-RR, Clone:240815E7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • ChIP experiment of K-562 cells using MITF Recombinant monoclonal antibody (83803-2-RR)

    ChIP experiment of K-562 using 83803-2-RR

    Chromatin was prepared from K-562 cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of MITF (83803-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

  • Affinity and Kinetic Characterization of 83803-2-RR

    Affinity and Kinetic Characterization of 83803-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83803-2-RR against Human MITF were performed. The affinity constant is 31.9 pM.

MITF Recombinant monoclonal antibody

MITF Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA, ChIP-qPCR
Cat No. 83803-2-RR
Clone No.240815E7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA, ChIP-qPCR

Synonyms

240815E7, bHLHe32, Class E basic helix-loop-helix protein 32, MI, Microphthalmia-associated transcription factor

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells, HepG2 cells, A431 cells
Positive FC (Intra) detected inHeLa cells
Positive ChIP-qPCR detected inK-562 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
CHIP-QPCRCHIP-QPCR : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83803-2-RR targets MITF in IF/ICC, FC (Intra), ELISA, ChIP-qPCR applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag3679

Product name: Recombinant human MITF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-91 aa of BC012503

Sequence: MLEMLEYNHYQVQTHLENPTKYHIQQAQRQQVKQYLSTTLANKHANQVLSLPCPNQPGDHVMPPVPGSSAPNSPMAMLTLNSNCEKEFMKQ

相同性解析による交差性が予測される生物種
Full Name microphthalmia-associated transcription factor
Calculated molecular weight 91 aa, 10 kDa, 59 kDa
Observed molecular weight 60-65 kDa
GenBank accession numberBC012503
Gene Symbol MITF
Gene ID (NCBI) 4286
RRIDAB_3671391
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO75030
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The retinal pigment epithelium (RPE) has a essential role in maintaining visual function and dedifferentiation of RPE contributes to the pathophysiology of several ocular diseases (PMID:22523078). Microphthalmia-associated transcription factor (MITF) is a key regulator of RPE differentiation that is also down-regulated in dedifferentiated hfRPE cells. MITF is a basic helix-loop-helix (hHLH)-leucine zipper protein that involves in the development of various cell types, including neural crest-derived melanocytes and optic cup-derived retinal pigment epithelial cells (PMID: 10578055).

Protocols

Product Specific Protocols
FC protocol for MITF antibody 83803-2-RRDownload protocol
IF protocol for MITF antibody 83803-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...