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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MITF Recombinant monoclonal antibody (83803-2-RR)

    IF Staining of HepG2 using 83803-2-RR (same clone as 83803-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MITF antibody (83803-2-RR, Clone: 240815E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83803-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using MITF Recombinant monoclonal antibody (83803-2-RR)

    IF Staining of A431 using 83803-2-RR (same clone as 83803-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using MITF antibody (83803-2-RR, Clone: 240815E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83803-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MITF Recombinant monoclonal antibody (83803-2-RR)

    IF Staining of HeLa using 83803-2-RR (same clone as 83803-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MITF antibody (83803-2-RR, Clone: 240815E7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83803-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using MITF Recombinant monoclonal antibody (83803-2-RR)

    FC experiment of HeLa using 83803-2-RR (same clone as 83803-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MITF Recombinant antibody (83803-2-RR, Clone:240815E7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83803-2-PBS in a different storage buffer formulation.

  • ChIP experiment of K-562 cells using MITF Recombinant monoclonal antibody (83803-2-RR)

    ChIP experiment of K-562 using 83803-2-RR (same clone as 83803-2-PBS)

    Chromatin was prepared from K-562 cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of MITF (83803-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 83803-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83803-2-PBS

    Affinity and Kinetic Characterization of 83803-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 83803-2-PBS against Human MITF was performed. The affinity constant is 3.09 nM.

  • Affinity and Kinetic Characterization of 83803-2-RR

    Affinity and Kinetic Characterization of 83803-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83803-2-RR against Human MITF were performed. The affinity constant is 31.9 pM.

MITF Recombinant monoclonal antibody, PBS Only

MITF Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA, ChIP-qPCR
Cat No. 83803-2-PBS
Clone No.240815E7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA, ChIP-qPCR

Synonyms

240815E7, bHLHe32, Class E basic helix-loop-helix protein 32, MI, Microphthalmia-associated transcription factor

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83803-2-PBS targets MITF in IF/ICC, FC (Intra), Indirect ELISA, ChIP-qPCR applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag3679

Product name: Recombinant human MITF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-91 aa of BC012503

Sequence: MLEMLEYNHYQVQTHLENPTKYHIQQAQRQQVKQYLSTTLANKHANQVLSLPCPNQPGDHVMPPVPGSSAPNSPMAMLTLNSNCEKEFMKQ

相同性解析による交差性が予測される生物種
Full Name microphthalmia-associated transcription factor
Calculated molecular weight 91 aa, 10 kDa, 59 kDa
Observed molecular weight 60-65 kDa
GenBank accession numberBC012503
Gene Symbol MITF
Gene ID (NCBI) 4286
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO75030
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The retinal pigment epithelium (RPE) has a essential role in maintaining visual function and dedifferentiation of RPE contributes to the pathophysiology of several ocular diseases (PMID:22523078). Microphthalmia-associated transcription factor (MITF) is a key regulator of RPE differentiation that is also down-regulated in dedifferentiated hfRPE cells. MITF is a basic helix-loop-helix (hHLH)-leucine zipper protein that involves in the development of various cell types, including neural crest-derived melanocytes and optic cup-derived retinal pigment epithelial cells (PMID: 10578055).