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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MIER2 Recombinant antibody (84216-1-RR)

    WB analysis using 84216-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84216-1-RR (MIER2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using MIER2 Recombinant antibody (84216-1-RR)

    IF Staining of A431 using 84216-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using MIER2 antibody (84216-1-RR, Clone: 241458D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84216-1-RR

    Affinity and Kinetic Characterization of 84216-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84216-1-RR against Human MIER2 were performed. The affinity constant is below 1 pM.

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MIER2 Recombinant antibody

MIER2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84216-1-RR
Clone No.241458D2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Mi-er2, Mesoderm induction early response protein 2, KIAA1193, 241458D2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU2OS cells, HEK-293 cells, Jurkat cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84216-1-RR targets MIER2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MIER2 fusion protein Ag11491 相同性解析による交差性が予測される生物種
Full Name mesoderm induction early response 1, family member 2
Calculated molecular weight 545 aa, 60 kDa
Observed molecular weight60 kDa
GenBank accession numberBC028203
Gene Symbol MIER2
Gene ID (NCBI) 54531
RRIDAB_3671772
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8N344
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mesoderm induction early response 2 (MIER2) is a member of the MIER family of proteins, which is relatively conservative in evolution and includes three members: MIER1, MIER2, and MIER3. The MIER family is known to contribute to histone deacetylase (HDAC) activity and actively participate in histone deacetylation (PMID: 28046085; PMID: 38702028). In renal cell carcinoma, MIER2 represses PGC1A expression by recruiting HDAC1 to deacetylate P53 (PMID: 28046085).

Protocols

Product Specific Protocols
WB protocol for MIER2 antibody 84216-1-RRDownload protocol
IF protocol for MIER2 antibody 84216-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols