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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MIER2 Recombinant antibody (84216-1-RR)

    WB analysis using 84216-1-RR (same clone as 84216-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84216-1-RR (MIER2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84216-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using MIER2 Recombinant antibody (84216-1-RR)

    IF Staining of A431 using 84216-1-RR (same clone as 84216-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using MIER2 antibody (84216-1-RR, Clone: 241458D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84216-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01148-1

    Cytometric bead array standard curve of MP01148-1

    Cytometric bead array standard curve of MP01148-1, MIER2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84216-2-PBS. Detection antibody: 84216-1-PBS. Standard: Ag11491. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP01148-2

    Cytometric bead array standard curve of MP01148-2

    Cytometric bead array standard curve of MP01148-2, MIER2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84216-3-PBS. Detection antibody: 84216-1-PBS. Standard: Ag11491. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84216-1-RR

    Affinity and Kinetic Characterization of 84216-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84216-1-RR against Human MIER2 were performed. The affinity constant is below 1 pM.

MIER2 Recombinant monoclonal antibody, PBS Only (Detector)

MIER2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 84216-1-PBS
Clone No.241458D2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

241458D2, KIAA1193, Mesoderm induction early response protein 2, Mi-er2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84216-1-PBS targets MIER2 as part of a matched antibody pair:

MP01148-1: 84216-2-PBS capture and 84216-1-PBS detection (validated in Cytometric bead array)

MP01148-2: 84216-3-PBS capture and 84216-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag11491

Product name: Recombinant human MIER2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 197-545 aa of BC028203

Sequence: IMVGPQFQADLSNLHLNRHCEKIYENEDQLLWDPSVLPEREVEEFLYRAVKRRWHEMAGPQLPEGEAVKDSEQALYELVKCNFNVEEALRRLRFNVKVIRDGLCAWSEEECRNFEHGFRVHGKNFHLIQANKVRTRSVGECVEYYYLWKKSERYDYFAQQTRLGRRKYVPSGTTDADQDLDGSDPDGPGRPRPEQDTLTGMRTDPLSVDGTAGGLDEPGVASDGLPSSEPGPCSFQQLDESPAVPLSHRPPALADPASYQPAVTAPEPDASPRLAVDFALPKELPLISSHVDLSGDPEETVAPAQVALSVTEFGLIGIGDVNPFLAAHPTCPAPGLHSEPLSHCNVMTC

相同性解析による交差性が予測される生物種
Full Name mesoderm induction early response 1, family member 2
Calculated molecular weight 545 aa, 60 kDa
Observed molecular weight60 kDa
GenBank accession numberBC028203
Gene Symbol MIER2
Gene ID (NCBI) 54531
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ8N344
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Mesoderm induction early response 2 (MIER2) is a member of the MIER family of proteins, which is relatively conservative in evolution and includes three members: MIER1, MIER2, and MIER3. The MIER family is known to contribute to histone deacetylase (HDAC) activity and actively participate in histone deacetylation (PMID: 28046085; PMID: 38702028). In renal cell carcinoma, MIER2 represses PGC1A expression by recruiting HDAC1 to deacetylate P53 (PMID: 28046085).

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