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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MIER2 Polyclonal antibody (17426-1-AP)

    WB analysis using 17426-1-AP (same clone as 17426-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17426-1-AP (MIER2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17426-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MIER2 Polyclonal antibody (17426-1-AP)

    WB analysis using 17426-1-AP (same clone as 17426-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17426-1-AP (MIER2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17426-1-PBS in a different storage buffer formulation.

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MIER2 Polyclonal antibody, PBS Only

MIER2 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 17426-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17426-1-PBS targets MIER2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MIER2 fusion protein Ag11491 相同性解析による交差性が予測される生物種
Full Name mesoderm induction early response 1, family member 2
Calculated molecular weight 545 aa, 60 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC028203
Gene Symbol MIER2
Gene ID (NCBI) 54531
RRIDAB_2143931
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N344
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Mesoderm induction early response 2 (MIER2) is a member of the MIER family of proteins, which is relatively conservative in evolution and includes three members: MIER1, MIER2, and MIER3. The MIER family is known to contribute to histone deacetylase (HDAC) activity and actively participate in histone deacetylation (PMID: 28046085; PMID: 38702028). In renal cell carcinoma, MIER2 represses PGC1A expression by recruiting HDAC1 to deacetylate P53 (PMID: 28046085).