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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MIER2 Polyclonal antibody (17426-1-AP)

    WB analysis using 17426-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17426-1-AP (MIER2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MIER2 Polyclonal antibody (17426-1-AP)

    WB analysis using 17426-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17426-1-AP (MIER2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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MIER2 Polyclonal antibody

MIER2 Polyclonal Antibody for WB, ELISA
Cat No. 17426-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Mi-er2, Mesoderm induction early response protein 2, KIAA1193

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, Jurkat cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17426-1-AP targets MIER2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MIER2 fusion protein Ag11491 相同性解析による交差性が予測される生物種
Full Name mesoderm induction early response 1, family member 2
Calculated molecular weight 545 aa, 60 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC028203
Gene Symbol MIER2
Gene ID (NCBI) 54531
RRIDAB_2143931
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N344
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mesoderm induction early response 2 (MIER2) is a member of the MIER family of proteins, which is relatively conservative in evolution and includes three members: MIER1, MIER2, and MIER3. The MIER family is known to contribute to histone deacetylase (HDAC) activity and actively participate in histone deacetylation (PMID: 28046085; PMID: 38702028). In renal cell carcinoma, MIER2 represses PGC1A expression by recruiting HDAC1 to deacetylate P53 (PMID: 28046085).

Protocols

Product Specific Protocols
WB protocol for MIER2 antibody 17426-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols