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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MID2 Monoclonal antibody (68359-1-Ig)

    WB analysis using 68359-1-Ig (same clone as 68359-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68359-1-Ig (MID2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68359-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using MID2 Monoclonal antibody (68359-1-Ig)

    WB analysis of Jurkat using 68359-1-Ig (same clone as 68359-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68359-1-Ig (MID2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68359-1-PBS in a different storage buffer formulation.

MID2 Monoclonal antibody, PBS Only

MID2 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68359-1-PBS
Clone No.1F9E4

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Synonyms

FLJ37715, FLJ41813, FXY2, MID2, Midin 2, midline 2, Midline defect 2, RING finger protein 60, RNF60, TRIM1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68359-1-PBS targets MID2 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag30793

Product name: Recombinant human MID2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 336-685 aa of BC017707

Sequence: AERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPHLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKTCH

相同性解析による交差性が予測される生物種
Full Name midline 2
Calculated molecular weight 715 aa, 81 kDa
Observed molecular weight80~83 kDa
GenBank accession numberBC017707
Gene Symbol MID2
Gene ID (NCBI) 11043
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9UJV3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Midline2 (MID2) is an ubiquitin-conjugating E2 enzyme that is involved in tumor development and has a physical interaction with breast cancer 1,early-onset (BRCA1).

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