Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, Jurkat cells, MCF-7 cells, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
68359-1-Ig targets MID2 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MID2 fusion protein Ag30793 相同性解析による交差性が予測される生物種 |
| Full Name | midline 2 |
| Calculated molecular weight | 715 aa, 81 kDa |
| Observed molecular weight | 80~83 kDa |
| GenBank accession number | BC017707 |
| Gene Symbol | MID2 |
| Gene ID (NCBI) | 11043 |
| RRID | AB_3085081 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9UJV3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Midline2 (MID2) is an ubiquitin-conjugating E2 enzyme that is involved in tumor development and has a physical interaction with breast cancer 1,early-onset (BRCA1).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MID2 antibody 68359-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncogenesis An E3 ligase TRIM1 promotes colorectal cancer progression via K63-linked ubiquitination and activation of HIF1α |