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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated MICAL2 Polyclonal an (CL488-13965)

    IF Staining of HepG2 using CL488-13965

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 MICAL2 antibody (CL488-13965) at dilution of 1:200.

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  • KD/KO Validated

CoraLite® Plus 488-conjugated MICAL2 Polyclonal antibody

MICAL2 Polyclonal Antibody for IF/ICC
Cat No. CL488-13965

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

DKFZp686H03148, DKFZp686H2469, KIAA0750, MICAL2, MICAL2PV1, MICAL2PV2, Protein MICAL 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-13965 targets MICAL2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MICAL2 fusion protein Ag4950 相同性解析による交差性が予測される生物種
Full Name microtubule associated monoxygenase, calponin and LIM domain containing 2
Calculated molecular weight 127 kDa
Observed molecular weight 95 kDa, 112 kDa
GenBank accession numberBC044577
Gene Symbol MICAL2
Gene ID (NCBI) 9645
RRIDAB_2919082
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94851
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MICALs (Molecules Interacting with CasL) are atypical multidomain flavoenzymes with diverse cellular functions.There are three known isoforms, MICAL1, MICAL2 and MICAL3, as well as the MICAL-like proteins MICAL-L1 and MICAL-L2. MICAL2 has three conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain and a Lin11, Isl-1 and Mec-3 (LIM) domain. It has been demonstrated that MICAL2 could regulate actin stress fibers and is required for normal actin organization. In addition, MICAL2-PV, a novel splicing variants of MICAL2, has been reported to be involved in cancer progression of prostate cancer. This antibody can recognize both MICAL2 and MICAL2-PV. This antibody recognizes various isoforms of MICAL2 around 90-95 kDa or 109-112 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MICAL2 antibody CL488-13965Download protocol
Standard Protocols
Click here to view our Standard Protocols