Validation Data Gallery
Tested Applications
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
APC-65161 targets MICA/MICB in FC applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Transfected C1R cells expressing MICA 相同性解析による交差性が予測される生物種 |
| Full Name | MHC class I polypeptide-related sequence A |
| Calculated molecular weight | 383 aa, 43 kDa |
| GenBank accession number | BC016929 |
| Gene Symbol | MICA |
| Gene ID (NCBI) | 100507436 |
| RRID | AB_2883034 |
| Conjugate | APC Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 650 nm / 660 nm |
| Form | |
| Form | Liquid |
| Purification Method | The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
| UNIPROT ID | Q29983 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). The monoclonal antibody 6D4 recognizes MICA and MICB.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for APC MICA/MICB antibody APC-65161 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
